4CO0
Structure of PII signaling protein GlnZ from Azospirillum brasilense in complex with adenosine diphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 |
| Unit cell lengths | 86.550, 86.550, 65.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.868 - 1.400 |
| R-factor | 0.2122 |
| Rwork | 0.210 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cny |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.178 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.870 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.080 | 0.520 |
| Number of reflections | 35124 | |
| <I/σ(I)> | 12.8 | 3.2 |
| Completeness [%] | 98.0 | 99.8 |
| Redundancy | 5.4 | 5.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1 MM MNCL2, 5MM 2OG, 5 MMATP, 0.2 M K/NA TARTRATE, 200% PEG 3350, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K, pH 7.5 |
