4CN6
GlgE isoform 1 from Streptomyces coelicolor E423A mutant with maltose bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-01-30 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 114.180, 114.180, 313.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.780 - 2.290 |
R-factor | 0.19618 |
Rwork | 0.195 |
R-free | 0.22003 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zss |
RMSD bond length | 0.011 |
RMSD bond angle | 1.351 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.780 | 2.350 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.090 | 0.580 |
Number of reflections | 93835 | |
<I/σ(I)> | 15.4 | 3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.7 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7.0 |