4CN1
GlgE isoform 1 from Streptomyces coelicolor D394A mutant with maltose- 1-phosphate bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 113.520, 113.520, 313.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.950 - 2.550 |
| R-factor | 0.18616 |
| Rwork | 0.185 |
| R-free | 0.21507 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zss |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.414 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.530 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.170 | 0.660 |
| Number of reflections | 67726 | |
| <I/σ(I)> | 11.4 | 3 |
| Completeness [%] | 99.7 | 96.6 |
| Redundancy | 14 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
