4CMZ
An intertwined homodimer of the PDZ homology domain of periaxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.840, 79.840, 81.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.920 - 2.700 |
| R-factor | 0.2049 |
| Rwork | 0.203 |
| R-free | 0.24100 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.994 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.040 | 0.780 |
| Number of reflections | 15882 | |
| <I/σ(I)> | 21 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% PEG 2000 MME, 0.15 M KBR, pH 7.5 |
