4CMP
Crystal structure of S. pyogenes Cas9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-31 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 159.780, 209.620, 91.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.481 - 2.620 |
| R-factor | 0.2532 |
| Rwork | 0.252 |
| R-free | 0.28580 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.948 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.520 | 2.690 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.050 | 0.640 |
| Number of reflections | 92408 | |
| <I/σ(I)> | 13.02 | 1.94 |
| Completeness [%] | 98.2 | 98.5 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS PH 8.5, 0.3 M LITHIUM SULFATE, 15% PEG 3350 |
