4CMM
Structure of human CD47 in complex with human Signal Regulatory Protein (SIRP) alpha v1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Collection date | 2010-06-12 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 67.390, 32.470, 69.610 |
| Unit cell angles | 90.00, 115.06, 90.00 |
Refinement procedure
| Resolution | 34.620 - 1.920 |
| R-factor | 0.1945 |
| Rwork | 0.193 |
| R-free | 0.22790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jjs |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS (XIA2) |
| Data scaling software | XSCALE (XIA2) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.620 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.050 | 0.440 |
| Number of reflections | 21067 | |
| <I/σ(I)> | 12.9 | 2.5 |
| Completeness [%] | 99.1 | 96 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 20% PEG 6000 0.1M TRIS PH8.5 |
