4CLW
Crystal structure of human soluble Adenylyl Cyclase in complex with alpha,beta-methyleneadenosine-5'-triphosphate soaked with bisulfite
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 63 |
| Unit cell lengths | 100.570, 100.570, 97.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.100 - 2.150 |
| R-factor | 0.16413 |
| Rwork | 0.162 |
| R-free | 0.20289 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4clf |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.870 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.100 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 58807 | |
| <I/σ(I)> | 20.3 | 4.8 |
| Completeness [%] | 98.0 | 97 |
| Redundancy | 10.3 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 100 MM PHOSPHATE/CITRATE PH 4.2, 200 MM NACL, 24 % (W/V) PEG 8000 |
