4CLA
ALTERNATIVE BINDING MODES FOR CHLORAMPHENICOL AND 1-SUBSTITUTED CHLORAMPHENICOL ANALOGUES REVEALED BY SITE-DIRECTED MUTAGENESIS AND X-RAY CRYSTALLOGRAPHY OF CHLORAMPHENICOL ACETYLTRANSFERASE
Experimental procedure
| Spacegroup name | H 3 2 |
| Unit cell lengths | 107.750, 107.750, 123.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.147 * |
| Rwork | 0.157 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 3.000 |
| Refinement software | DERIV |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 6.3 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | buttom | protein | 50 (mg/ml) | |
| 2 | 1 | buttom | MES | 10 (mM) | |
| 3 | 1 | reservoir | chloramphenicol | 1 (mM) | |
| 4 | 1 | reservoir | MPD | 5 (%) | |
| 5 | 1 | reservoir | MES | 10 (mM) | |
| 6 | 1 | reservoir | hexammine cobalt(III) chloride | 0.5 (mM) |
