4CJM
Crystal structure of human FGF18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.760, 49.496, 100.854 |
| Unit cell angles | 90.00, 101.74, 90.00 |
Refinement procedure
| Resolution | 98.740 - 2.700 |
| R-factor | 0.21344 |
| Rwork | 0.212 |
| R-free | 0.25083 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fdb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.326 |
| Data reduction software | PROTEUM2 |
| Data scaling software | PROTEUM2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.740 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.190 | |
| Number of reflections | 17815 | |
| <I/σ(I)> | 15.84 | 2.83 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.3 | 12.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES PH 6.5, 0.2 M (NH4)2SO4 AND 26% PEG 5000 |
