4CJ6
Crystal structure of the complex of the Cellular Retinal Binding Protein Mutant R234W with 9-cis-retinal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-11-03 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.870, 57.918, 75.206 |
| Unit cell angles | 90.00, 122.88, 90.00 |
Refinement procedure
| Resolution | 44.745 - 1.896 |
| R-factor | 0.1683 |
| Rwork | 0.165 |
| R-free | 0.19750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hx3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.841 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.340 |
| Number of reflections | 19606 | |
| <I/σ(I)> | 14.48 | 4.12 |
| Completeness [%] | 98.4 | 92.9 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
