4CIU

Crystal structure of E. coli ClpB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-07
Detector	ADSC QUANTUM 315r
Spacegroup name	P 65
Unit cell lengths	127.329, 127.329, 119.852
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.800 - 3.500
R-factor	0.2252
Rwork	0.224
R-free	0.24700
Structure solution method	SAD
Starting model (for MR)	NONE
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.200	3.690
High resolution limit [Å]	3.500	3.500
Rmerge	0.090	0.530
Number of reflections	14024
<I/σ(I)>	16.8	5.4
Completeness [%]	99.9	100
Redundancy	14.5	14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			291	1.5 M AMSO4, 0.4 % PEG 400, 0.1 M HEPES PH 7.5, 22 % GLYCEROL AT 291 K