4CIS
Structure of MutM in complex with carbocyclic 8-oxo-G containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.063, 112.692, 132.882 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.930 - 2.050 |
| R-factor | 0.21507 |
| Rwork | 0.214 |
| R-free | 0.23885 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xc8 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.821 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.930 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.150 | 1.180 |
| Number of reflections | 41439 | |
| <I/σ(I)> | 10.73 | 1.5 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 5.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM TRIS PH 7.5, 2 MM SPERMIDINE, 1 MM TCEP, 0.01MM ZNCL2 , 90 MM LISO4, 18% PEG 4000 |
