4CII

Crystal structure of N-terminally truncated Helicobacter pylori T4SS Protein CagL as domain swapped dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-24
Detector	ADSC QUANTUM 315r
Spacegroup name	P 65 2 2
Unit cell lengths	61.841, 61.841, 244.499
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.282 - 2.150
R-factor	0.2003
Rwork	0.199
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3CZI
RMSD bond length	0.009
RMSD bond angle	0.968
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.750	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.080	0.500
Number of reflections	16080
<I/σ(I)>	15.7	3.2
Completeness [%]	99.8	99.5
Redundancy	9	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.2	277	100 MM CITRATE/PHOSPHATE PH 4.2, 40% (V/V) 2-METHYL-2,4-PENTANEDIOL, 6.4% TERT-BUTANOL. VAPOR DIFFUSION AT 277 K WITH DROP RATIO OF 2:1 PROTEIN TO RESERVOIR.