4CHF
Crystal structure of the putative cap-binding domain of the PB2 subunit of Thogoto virus polymerase (form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 101.440, 101.440, 106.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.982 - 3.000 |
| R-factor | 0.2004 |
| Rwork | 0.200 |
| R-free | 0.21580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | THOGOTO PB2 PUTATIVE CAP-BINDING DOMAIN FORM 1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.846 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.220 | 0.950 |
| Number of reflections | 11610 | |
| <I/σ(I)> | 12.9 | 3.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.2 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 100 MM BIS-TRIS PH 6.0, 100 MM MGCL2 AND 22 % PEG3350 |
