4CH9
Crystal structure of the human KLHL3 Kelch domain in complex with a WNK4 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.330, 84.760, 146.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.402 - 1.840 |
| R-factor | 0.1459 |
| Rwork | 0.144 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xn4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.178 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.400 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.060 | 0.250 |
| Number of reflections | 49110 | |
| <I/σ(I)> | 14.4 | 4 |
| Completeness [%] | 98.2 | 86.3 |
| Redundancy | 4.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.3 | 293 | SITTING-DROP VAPOUR-DIFFUSTION, 25-35 % PEG 4000, 0.2 M AMMONIUM SULPHATE, 0.1 M ACETATE (PH 4.3), 293 K |
