4CGS
Crystal structure of the N-terminal domain of the PA subunit of Dhori virus polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.910, 87.450, 56.130 |
| Unit cell angles | 90.00, 112.98, 90.00 |
Refinement procedure
| Resolution | 51.680 - 1.300 |
| R-factor | 0.17509 |
| Rwork | 0.173 |
| R-free | 0.21964 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.928 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.060 | 0.370 |
| Number of reflections | 92519 | |
| <I/σ(I)> | 14.8 | 4.3 |
| Completeness [%] | 96.6 | 94.2 |
| Redundancy | 3.41 | 3.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20MG/ML PROTEIN, 3.2 M NA FORMATE AND 0.1 M HEPES PH 7.5 |
