4CEM
Crystal structure of the first MIF4G domain of human nonsense mediated decay factor UPF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 101.080, 101.080, 158.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.540 - 2.600 |
| R-factor | 0.20761 |
| Rwork | 0.205 |
| R-free | 0.25087 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.059 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD (SHARP) |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.090 | 0.630 |
| Number of reflections | 28216 | |
| <I/σ(I)> | 10.9 | 1.7 |
| Completeness [%] | 99.1 | 99.3 |
| Redundancy | 2.86 | 2.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 100 MM BICINE PH 9, 100 MM NACL, 11% PEG 6000 AND AT A PROTEIN CONCENTRATION OF 16 MG/ML |
