4CEJ
Crystal structure of AddAB-DNA-ADPNP complex at 3 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.437, 152.941, 125.244 |
| Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
| Resolution | 29.731 - 3.000 |
| R-factor | 0.2048 |
| Rwork | 0.203 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u44 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.774 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (PHASER-MR) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.550 | 3.090 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.050 | 0.420 |
| Number of reflections | 57963 | |
| <I/σ(I)> | 18.4 | 2.9 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M TRIS-HCL PH 7.5, 11% PEG 4000, 0.1M MAGNESIUM ACETATE |
