4CE2
Hsp90 N-terminal domain bound to macrolactam analogues of radicicol.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 74.320, 74.320, 110.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.460 - 2.380 |
| R-factor | 0.19527 |
| Rwork | 0.193 |
| R-free | 0.23936 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ah6 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.848 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.460 | 2.450 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.110 | 0.800 |
| Number of reflections | 12939 | |
| <I/σ(I)> | 14.2 | 2.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.5 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
