4CDO

Crystal structure of PQBP1 bound to spliceosomal U5-15kD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Collection date	2011-12-07
Spacegroup name	P 1 21 1
Unit cell lengths	59.360, 53.700, 66.190
Unit cell angles	90.00, 89.95, 90.00

Refinement procedure

Resolution	33.095 - 2.500
R-factor	0.1895
Rwork	0.187
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.054
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.820	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.160	0.640
Number of reflections	14640
Completeness [%]	100.0	100
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1