4CD5
The structure of GH26 beta-mannanase CjMan26C from Cellvibrio japonicus in complex with ManMIm
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 84.507, 84.507, 244.941 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 73.190 - 1.100 |
| R-factor | 0.15273 |
| Rwork | 0.152 |
| R-free | 0.17213 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vx7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.549 |
| Data reduction software | xia2 (IMPLEMENTATION OF XDS) |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.100 | |
| Number of reflections | 208589 | |
| <I/σ(I)> | 11.7 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.3 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 100MM SODIUM CITRATE PH 5.6, 15% PEG 3000 |
