4CCV
Crystal structure of histidine-rich glycoprotein N2 domain reveals redox activity at an interdomain disulfide bridge: Implications for the regulation of angiogenesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-26 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 77.138, 77.138, 69.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.710 - 1.930 |
| R-factor | 0.18671 |
| Rwork | 0.185 |
| R-free | 0.22524 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.019 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.710 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.050 | 0.330 |
| Number of reflections | 17163 | |
| <I/σ(I)> | 11.3 | 2.1 |
| Completeness [%] | 93.2 | 69.2 |
| Redundancy | 4.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 22.5% PEG MME 5000, 0.073M POTASSIUM SODIUM TARTRATE, 0.1M MES PH6.5 |
