4CC7
Crystal structure of the sixth or C-terminal SH3 domain of human Tuba in complex with proline-rich peptides of N-WASP. Space group P41
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 |
| Unit cell lengths | 88.570, 88.570, 69.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.590 - 1.970 |
| R-factor | 0.17798 |
| Rwork | 0.176 |
| R-free | 0.21459 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zuu |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.038 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.050 | 0.710 |
| Number of reflections | 38387 | |
| <I/σ(I)> | 20.7 | 2.6 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 3 M NACL, 0.1 M BIS-TRIS PH 5.5 |
