4CBL
Pestivirus NS3 helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-04-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.910, 156.840, 99.400 |
| Unit cell angles | 90.00, 97.21, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.050 |
| R-factor | 0.1959 |
| Rwork | 0.194 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cbg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.210 | 3.210 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.120 | 0.640 |
| Number of reflections | 38441 | |
| <I/σ(I)> | 10.4 | 1.8 |
| Completeness [%] | 94.9 | 73.3 |
| Redundancy | 5.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M NA-CACODYLATE, 0.15 M CALCIUM ACETATE, 12% PEG 3350, pH 6.5 |
