4CBK
The c-ring ion binding site of the ATP synthase from Bacillus pseudofirmus OF4 is adapted to alkaliphilic cell physiology
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.790, 96.750, 118.330 |
| Unit cell angles | 90.00, 105.38, 90.00 |
Refinement procedure
| Resolution | 40.004 - 2.420 |
| R-factor | 0.1966 |
| Rwork | 0.195 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x2v |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.793 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.500 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.170 | 0.970 |
| Number of reflections | 60954 | |
| <I/σ(I)> | 12.33 | 1.84 |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 4.53 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | pH 9 |
