4CBA
Structural of delta 1-76 CTNNBL1 in space group I222
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 87.422, 92.848, 194.949 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.920 - 3.100 |
| R-factor | 0.21953 |
| Rwork | 0.216 |
| R-free | 0.27846 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cb8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.744 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.300 | 3.300 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.100 | 0.400 |
| Number of reflections | 14575 | |
| <I/σ(I)> | 10.5 | 3.6 |
| Completeness [%] | 98.8 | 98.4 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.12M ETHYLENE GLYCOL, 12.5% PEG 3350, 12.5% PEG 1K, 12.5% MPD, 0.1 M BICINE/TRIZMA BASE |
