4CB7
Structure of Influenza A H5N1 PB2 cap-binding domain with bound cap analogue (compound 8e)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-30 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.940, 108.180, 39.050 |
| Unit cell angles | 90.00, 91.08, 90.00 |
Refinement procedure
| Resolution | 36.960 - 1.850 |
| R-factor | 0.17336 |
| Rwork | 0.171 |
| R-free | 0.21410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vqz |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.630 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.070 | 0.430 |
| Number of reflections | 25819 | |
| <I/σ(I)> | 12.3 | 2.8 |
| Completeness [%] | 98.9 | 99.7 |
| Redundancy | 3.29 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 0.2M AMMONIUM NITRATE, PH 6.3, 20 % PEG 3350 |
