4CB5
Structure of Influenza A H5N1 PB2 cap-binding domain with bound cap analogue (compound 8f)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 65 |
| Unit cell lengths | 82.170, 82.170, 54.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.480 - 1.500 |
| R-factor | 0.12822 |
| Rwork | 0.127 |
| R-free | 0.15850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vqz |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.913 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.560 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.480 |
| Number of reflections | 33681 | |
| <I/σ(I)> | 16.5 | 2.9 |
| Completeness [%] | 99.4 | 98.9 |
| Redundancy | 3.68 | 3.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.3 | 1M SODIUM ACETATE PH 4.3, 2.4M NACL |
