4CAH

Structure of inner DysF domain of human dysferlin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-05
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 3
Unit cell lengths	75.953, 75.953, 75.953
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.707 - 1.901
R-factor	0.1781
Rwork	0.177
R-free	0.19080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2k2o
RMSD bond length	0.015
RMSD bond angle	1.764
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MrBUMP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.700	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.040	0.670
Number of reflections	11803
<I/σ(I)>	30.5	3.5
Completeness [%]	100.0	100
Redundancy	9.8	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		0.04M POTASSIUM DIHYDROGEN PHOSPHATE, 16% PEG 8000, 20% GLYCEROL, 0.2M NABR, pH 7.5