4C9B
Crystal structure of eIF4AIII-CWC22 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.290, 108.720, 61.370 |
| Unit cell angles | 90.00, 99.94, 90.00 |
Refinement procedure
| Resolution | 55.445 - 2.000 |
| R-factor | 0.2035 |
| Rwork | 0.202 |
| R-free | 0.23580 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.872 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.400 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.640 |
| Number of reflections | 48938 | |
| <I/σ(I)> | 12.68 | 2.17 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 5.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% PEG 20000, 0.1 M NA-K-PHOSPHATE PH 6.5 |
