4C6B
Crystal structure of the dihydroorotase domain of human CAD with incomplete active site, obtained recombinantly from E. coli.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.720, 160.120, 60.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.858 - 1.656 |
| R-factor | 0.1251 |
| Rwork | 0.123 |
| R-free | 0.17270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4by3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.198 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.020 | 1.770 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.050 | 0.460 |
| Number of reflections | 41707 | |
| <I/σ(I)> | 22.4 | 2.3 |
| Completeness [%] | 89.3 | 52.4 |
| Redundancy | 5.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6 |
