4C69
ATP binding to murine voltage-dependent anion channel 1 (mVDAC1).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-30 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.152, 58.369, 66.545 |
| Unit cell angles | 90.00, 99.25, 90.00 |
Refinement procedure
| Resolution | 19.497 - 2.277 |
| R-factor | 0.2066 |
| Rwork | 0.204 |
| R-free | 0.26110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3emn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.109 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | 0.700 |
| Number of reflections | 16915 | |
| <I/σ(I)> | 23.13 | 2.21 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 3.64 | 3.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 18-20% MPD, 0.1 M TRISHCL (PH 8.5), 10% PEG400 |
