4C5O
Flavin monooxygenase from Stenotrophomonas maltophilia. Q193R H194T mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2012-07-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 32 |
| Unit cell lengths | 170.538, 170.538, 101.762 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 147.690 - 2.600 |
| R-factor | 0.257 |
| Rwork | 0.255 |
| R-free | 0.29496 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a9w |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.656 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0033) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.780 | 2.650 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.150 | 0.560 |
| Number of reflections | 102301 | |
| <I/σ(I)> | 4.6 | 2.1 |
| Completeness [%] | 97.8 | 99.4 |
| Redundancy | 4.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | THE RESERVOIR CONTAINED 0.9 M LITHIUM SULPHATE IN 100 MM BIS-TRIS PROPANE BUFFER (PH 5.6). THE PROTEIN CONCENTRATION USED WAS 3 MG ML-1. |
