4C58
Structure of GAK kinase in complex with nanobody (NbGAK_4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-11 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.866, 37.430, 76.010 |
| Unit cell angles | 90.00, 108.48, 90.00 |
Refinement procedure
| Resolution | 38.380 - 2.160 |
| R-factor | 0.18241 |
| Rwork | 0.179 |
| R-free | 0.24172 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3LL6 AND 1OP9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.596 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.090 | 2.280 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.110 | 0.780 |
| Number of reflections | 22748 | |
| <I/σ(I)> | 10.3 | 2 |
| Completeness [%] | 99.2 | 99.7 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 12% PEG 3350, 0.13 M MAGNESIUM FORMATE, pH 7.5 |
