4C57
Structure of GAK kinase in complex with a nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.160, 86.660, 83.910 |
| Unit cell angles | 90.00, 96.44, 90.00 |
Refinement procedure
| Resolution | 74.680 - 2.550 |
| R-factor | 0.1802 |
| Rwork | 0.178 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3LL6 AND 1OP9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.390 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.090 | 0.640 |
| Number of reflections | 34717 | |
| <I/σ(I)> | 10.5 | 2.1 |
| Completeness [%] | 99.1 | 95.1 |
| Redundancy | 5.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 14% PEG 3350, 0.2 M SODIUM SULFATE, 0.1 M BIS-TRIS PROPANE, PH 7.0, 10% ETHYLENE GLYCOL |
