4C54

Crystal structure of recombinant human IgG4 Fc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-10
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	74.837, 78.973, 97.881
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.941 - 1.900
R-factor	0.172
Rwork	0.170
R-free	0.20860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1adq
RMSD bond length	0.013
RMSD bond angle	1.490
Data reduction software	XDS (IN XIA2 PACKAGE)
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.800	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.110
Number of reflections	46409
<I/σ(I)>	16.7	2.1
Completeness [%]	99.9	99.9
Redundancy	14.4	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		MES PH6.5. 18-20% PEG 20 000