4C30
Crystal structure of Deinococcus radiodurans UvrD in complex with DNA, form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.489, 89.785, 293.798 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 66.180 - 3.000 |
| R-factor | 0.23114 |
| Rwork | 0.228 |
| R-free | 0.28783 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DRUVRD FORM1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.544 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0085) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.200 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.060 | 0.320 |
| Number of reflections | 62649 | |
| <I/σ(I)> | 6.5 | 1.9 |
| Completeness [%] | 89.0 | 86.5 |
| Redundancy | 1.5 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 22% PEG 3350, 0.1 M BIS-TRIS PROPANE PH 7.5, 0.1 M NA-FLUORIDE |
