4C2X
Human N-myristoyltransferase isoform 2 (NMT2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-11 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.700, 72.320, 114.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.170 - 2.330 |
| R-factor | 0.22173 |
| Rwork | 0.218 |
| R-free | 0.28812 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iu1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.672 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0033) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 2.410 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.060 | 0.640 |
| Number of reflections | 18414 | |
| <I/σ(I)> | 15.2 | 2.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.3 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 25% PEG 3350, 0.2M KCL, 100 MM NA CITRATE PH 5.5 |
