4C2U

Crystal structure of Deinococcus radiodurans UvrD in complex with DNA, Form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	67.572, 67.448, 386.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.740 - 2.550
R-factor	0.2141
Rwork	0.211
R-free	0.26651
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pjr
RMSD bond length	0.017
RMSD bond angle	1.744
Data scaling software	SCALA
Phasing software	MrBUMP
Refinement software	REFMAC (5.6.0085)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.400	2.690
High resolution limit [Å]	2.550	2.550
Rmerge	0.070	0.590
Number of reflections	56088
<I/σ(I)>	20.4	3.6
Completeness [%]	100.0	100
Redundancy	7.3	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				20% PEG 3350, 0.1 M BIS-TRIS PROPANE PH 7.0, 0.2 M NA-NITRATE