4C2K
Crystal structure of human mitochondrial 3-ketoacyl-CoA thiolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-17 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.730, 196.770, 79.650 |
Unit cell angles | 90.00, 103.78, 90.00 |
Refinement procedure
Resolution | 48.170 - 2.000 |
R-factor | 0.19695 |
Rwork | 0.195 |
R-free | 0.22889 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dm3 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.509 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.170 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.410 |
Number of reflections | 109515 | |
<I/σ(I)> | 15.07 | 3.07 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.6 | 100 MM MES PH 6.6, 15% MME-PEG5000 |