4C25

L-fuculose 1-phosphate aldolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-01
Detector	DECTRIS PILATUS 6M
Spacegroup name	I 4
Unit cell lengths	113.490, 113.490, 41.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.250 - 2.030
R-factor	0.22617
Rwork	0.223
R-free	0.27799
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fua
RMSD bond length	0.012
RMSD bond angle	1.431
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.140
High resolution limit [Å]	2.030	2.030
Rmerge	0.120	0.250
Number of reflections	15800
<I/σ(I)>	6.5	3.1
Completeness [%]	97.7	98.1
Redundancy	4.5	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		SPFCSA AT A CONCENTRATION OF 14 MG PER ML, BUFFERED IN 20 MM TRIS-HCL (PH 8.0) AND 5 MM DITHIOTHREITOL (DTT) WAS CRYSTALLIZED BY MIXING EQUAL VOLUMES WITH A SOLUTION OF 4% (V PER V) GLYCEROL, 1.50 M AMMONIUM SULPHATE, 100 MM TRIS-HCL (PH 8.5) AND 8 MM ZINC CHLORIDE.