4C1Y
Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with b-methylfucoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.211, 90.335, 189.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.140 - 2.230 |
| R-factor | 0.24611 |
| Rwork | 0.243 |
| R-free | 0.31339 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4agi |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.495 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.290 | 2.290 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.130 | 0.350 |
| Number of reflections | 56864 | |
| <I/σ(I)> | 6.14 | |
| Completeness [%] | 99.0 | 97.8 |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M CACL2, 25% PEG4K, 0.1M TRIS, PH 8.0 |
