4C0N
Crystal structure of non symbiotic plant hemoglobin AHb3 (GLB3) from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-31 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 123.373, 123.373, 123.373 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.420 - 1.770 |
| R-factor | 0.18675 |
| Rwork | 0.186 |
| R-free | 0.20530 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.510 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.300 | 1.810 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.050 | 0.770 |
| Number of reflections | 31454 | |
| <I/σ(I)> | 16.4 | 1.9 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 1.6 M AMMONIUM SULFATE, 0.1 M TRIS PH 8.2 |
