4C0K

Crystal structure of Drosophila Miro EF hand and cGTPase domains bound to one calcium ion (Ca-MiroS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-29
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 32 2 1
Unit cell lengths	82.240, 82.240, 156.441
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.075 - 2.801
R-factor	0.2107
Rwork	0.207
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	INITIAL MODEL FROM PRIOR DATASET
RMSD bond length	0.003
RMSD bond angle	0.475
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.100	2.970
High resolution limit [Å]	2.800	2.800
Rmerge	0.140	0.700
Number of reflections	15693
<I/σ(I)>	11.1	1.6
Completeness [%]	100.0	100
Redundancy	15.1	15.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		5.0MG/ML MIROS, 1.7 M LISO4, 0.1 M BIS-TRIS PH 7.4, 5 MM EGTA; CRYSTAL SOAKED IN 10MM CACL2 FOR 72H PRIOR TO DATA COLLECTION.