4C0J
Crystal structure of Drosophila Miro EF hand and cGTPase domains in the apo state (Apo-MiroS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.930, 81.930, 155.821 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.590 - 2.820 |
| R-factor | 0.2285 |
| Rwork | 0.225 |
| R-free | 0.26170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | INITIAL MODEL FROM PRIOR DATASET |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.524 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 2.890 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.100 | 0.590 |
| Number of reflections | 15216 | |
| <I/σ(I)> | 16.6 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.6 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 5.0MG/ML MIROS, 1.7M LISO4, 0.1M BIS-TRIS PH 7.6, 5MM EGTA |
