4C0F
Structure of the NOT-box domain of human CNOT2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-11-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00001,0.97945 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 64.830, 64.830, 412.290 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.304 - 2.400 |
R-factor | 0.2193 |
Rwork | 0.217 |
R-free | 0.26280 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.002 |
RMSD bond angle | 0.641 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.300 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 21312 | |
<I/σ(I)> | 13.2 | 2.4 |
Completeness [%] | 99.4 | 99.1 |
Redundancy | 6.7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 100MM TRI-SODIUM CITRATE PH=5.6, 10% PEG4000, 10% ISOPROPANOL |