4C05

Crystal structure of M. musculus protein arginine methyltransferase PRMT6 with SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-11-09
Detector	DECTRIS PILATUS 6M
Spacegroup name	I 41
Unit cell lengths	78.427, 78.427, 118.126
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.428 - 2.195
R-factor	0.1681
Rwork	0.146
R-free	0.18500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y1w
RMSD bond length	0.009
RMSD bond angle	1.043
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.430	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.080	0.880
Number of reflections	17706
<I/σ(I)>	13.46	1.37
Completeness [%]	97.0	78.6
Redundancy	4.08	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		pH 7.0