4C03
Crystal structure of M. musculus protein arginine methyltransferase PRMT6 reduced
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 118.722, 143.340, 41.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.560 - 1.580 |
| R-factor | 0.1617 |
| Rwork | 0.160 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y1w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.281 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.560 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.110 | 1.010 |
| Number of reflections | 98190 | |
| <I/σ(I)> | 8.71 | 0.99 |
| Completeness [%] | 99.0 | 90.7 |
| Redundancy | 6.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 100 MM HEPES PH 7.0, 200 MM MGCL2, 25% PEG 1500 |
