4C00

Crystal structure of TamA from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-12
Detector	DECTRIS PILATUS 2M
Spacegroup name	P 21 21 2
Unit cell lengths	77.470, 261.060, 57.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.269 - 2.250
R-factor	0.1907
Rwork	0.190
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2QDZ AND 4BZA
RMSD bond length	0.003
RMSD bond angle	0.956
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.000	2.390
High resolution limit [Å]	2.250	2.250
Rmerge	0.070	0.850
Number of reflections	55995
<I/σ(I)>	20.74	2.39
Completeness [%]	98.4	94.3
Redundancy	7.29	7.04

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				IMIDAZOLE PH 6.0, NA ACETATE, TRIS, NACL, BETA-OG, DMPC, CHAPSO