4BZH

Crystal structure of galactose mutarotase GalM from Bacillus subtilis in complex with maltose and trehalose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-01-29
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 2
Unit cell lengths	82.194, 83.291, 124.754
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.430 - 2.600
R-factor	0.21677
Rwork	0.214
R-free	0.26904
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mwx
RMSD bond length	0.009
RMSD bond angle	1.381
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.430	2.480
High resolution limit [Å]	2.350	2.350
Rmerge	0.280	0.500
Number of reflections	36065
<I/σ(I)>	4.4	2.3
Completeness [%]	99.0	93.2
Redundancy	6.5	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1